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contacting the first fusion protein with the second fusion protein in binding buffer, wherein the second fusion protein is attached to a resin that binds to the purification tag, wherein the purification tag is capable of specifically binding a purification resin, wherein a complex between the first fusion protein and the second fusion protein is formed, wherein the binding buffer inhibits a the invention includes embodiments of fusion proteins comprising a protein of interest (POI) and an intein C-fragment, wherein the POI is fused to the C-terminus of the intein C-fragment, wherein the intein is a naturally split intein DnaE, and the intein C-fragment carries a Asp118Gly mutation within the intein C-fragment

Dietary glutamine and oral antibiotics each improve indexes of gut barrier function in rat short bowel syndrome

Cell 115 , 281292 (2003)

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